Anti-MBD2 Rabbit Monoclonal Antibody

MBD2 antibody

Boster Bio Anti-MBD2 Rabbit Monoclonal Antibody catalog # M01746. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01746
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-MBD2 Rabbit Monoclonal Antibody

View all MBD2 Antibodies

SKU/Catalog Number

M01746

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MBD2 Rabbit Monoclonal Antibody catalog # M01746. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MBD2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01746)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADIH-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human MBD2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01746 is reactive to MBD2 in Human, Mouse, Rat

Applications

M01746 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

43.255kDa

Background of MBD2

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For MBD2 (Source: Uniprot.org, NCBI)

Gene Name

MBD2

Full Name

Methyl-CpG-binding domain protein 2

Weight

43.255kDa

Alternative Names

demethylase; DKFZp586O0821; DMTase; methyl-CpG binding domain protein 2; methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY-CO-41 MBD2 DMTase, NY-CO-41 methyl-CpG binding domain protein 2 methyl-CpG-binding domain protein 2|demethylase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MBD2, check out the MBD2 Infographic

MBD2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MBD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-MBD2 Rabbit Monoclonal Antibody

Question

What sample was tested in WB and IHC applications with M01746?

Verified customer

Asked: 2020-01-09

Answer

Western blot analysis of MBD2 expression in A375 cell lysate.

Boster Scientific Support

Answered: 2020-01-09

Question

We need to test anti-MBD2 Monoclonal antibody M01746 on mouse testis for research purposes, then I may be interested in using anti-MBD2 Monoclonal antibody M01746 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

The products we sell, including anti-MBD2 Monoclonal antibody M01746, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-30

Question

Would anti-MBD2 Monoclonal antibody M01746 work for WB with testis?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

According to the expression profile of testis, MBD2 is highly expressed in testis. So, it is likely that anti-MBD2 Monoclonal antibody M01746 will work for WB with testis.

Boster Scientific Support

Answered: 2019-10-15

Question

Is this M01746 anti-MBD2 Monoclonal antibody reactive to the isotypes of MBD2?

J. Johnson

Verified customer

Asked: 2017-12-27

Answer

The immunogen of M01746 anti-MBD2 Monoclonal antibody is A synthesized peptide derived from human MBD2 Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-27

Question

Would M01746 anti-MBD2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

O. Li

Verified customer

Asked: 2017-01-24

Answer

As indicated on the product datasheet, M01746 anti-MBD2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-01-24

Question

Is a blocking peptide available for product anti-MBD2 Monoclonal antibody (M01746)?

T. Banerjee

Verified customer

Asked: 2015-06-12

Answer

We do provide the blocking peptide for product anti-MBD2 Monoclonal antibody (M01746). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-06-12

Order DetailsPrice
M01746

100μL

$370
M01746-30ul

30μL sample

$99
M01746-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M01746
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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